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KMID : 0385019930090010017
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Korean Journal of Laboratory Animal Science
1993 Volume.9 No. 1 p.17 ~ p.29
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A Field Survey by Means of the Indirect Fluorescent Antibody Technique for Detection and Diagnosis of Pathogenic Bacteria in Freshwater Fishes
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Abstract
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In this study, we described a diagnostic system based on indirect fluorescent antibody technique(IFAT) for detection of bacterial diseases in cultured freshwater fishes. Rabbit antisera of 3 bacterial pathogens(Flavobacterium branchiophila, Aeromonas hydrophila, Aeromonas salmonicida) and protein A-FITC conjugate are prepared. The lesions from experimentally or naturally infected fishes which were selected randomly and infected in some aquafarms investigated comparing IFAT to standard culture method. Results revealed that the sensitivity and specificity of IFAT were capable of detecting the bacterial pathogens from the hosts and the environment.
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KEYWORD
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